• Übersicht

  Artikelnummer	115-035-003
  Wirtsspezies	Ziege
  IgG-Form	IgG Gesamtmolekül
  Spezies-Reaktivität	Maus
  Spezifität	IgG (H+L)
  Isotyp	Ig
  Klonalität (Mono-/Polyklonal)	polyklonal
  Anwendung	ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot
  Konjugation	Horseradish Peroxidase (HRP)
  Keine Kreuzreaktivität (MinX) mit	keine
  Verdünnung	ELISA 1:5.000 – 1:100.000, Histo-/Zytochemie 1:500 – 1:5.000, Western Blot (ECL): 1:10.000 – 1:200.000, Western Blot (non-ECL): 1:5.000 – 1:100.000
  Format	15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat
  Zweckbestimmung	nur für Forschungszwecke
  Produktlinie / Thema	Jackson ImmunoResearch
  Hersteller / Marke	Jackson ImmunoResearch

• Datenblätter und Downloads

• 115-035-003 – Datenblatt

• Weitere Produktinformationen

  Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

  
  

  Conjugate

  Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度，再用二抗稀释液稀释至该二抗的建议稀释范围。