Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Alexa Fluor® 680
Amax: 684Emax: 702nmAlexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.
Jackson冻干粉二抗如何配置
先加入蒸馏水稀释至Jackson二抗说明书所述浓度，再用二抗稀释液稀释至该二抗的建议稀释范围。
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江浙沪皖
