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主营:Jackson、二抗、血清、驴血清以及免疫球蛋白的生产和偶联
℡ 4000-520-616
℡ 4000-520-616
Jackson/Alkaline Phosphatase IgG Fraction Monoclonal Mouse Anti-Human IgE (ME.114)/0.5 ml/209-052-241
产品编号:209-052-241
市  场 价:¥5000.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>其他>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$250.00
品      牌: Jackson
公司分类:
Jackson/Alkaline Phosphatase IgG Fraction Monoclonal Mouse Anti-Human IgE (ME.114)/0.5 ml/209-052-241
商品介绍
Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with human IgE. No antibody was detected against human IgG, IgM, or IgA. The antibody may cross-react with immunoglobulins from other species.Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The IgG fraction was purified by Protein A chromatography. Buffer: 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:

ELISA:- 1:5,000-1:50,000 Western Blot:- 1:5,000-1:50,000 Not recommended for detection of reduced IgE

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Alkaline Phosphatase

Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.

Images & References

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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