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Jackson/Alexa Fluor® 647 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific/1.5 mg/112-605-008

  • 产品编号: 112-605-008
  • 美  元  价: $158.00
  • 市  场  价: ¥3160.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司:
  • 产品分类:
  • 公司分类: Whole IgG Affinity-Purified Antibodies
Jackson/Alexa Fluor® 647 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific/1.5 mg/112-605-008
  • Jackson/Alexa Fluor® 647 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific/1.5 mg/112-605-008
商品介绍

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins.No antibody was detected against rat IgM or against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Alexa Fluor® 647

Amax: 651Emax: 667nm

Alexa Fluor® 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. Alexa Fluor® 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. Alexa Fluor® 647 conjugates are the best choice of far red-emitting dyes for multiple-labeling detection with a confocal microscope.A significant advantage of using Alexa Fluor® 647 over lower wavelength-emitting dyes is the low autofluorescence of biological specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor® 647 is not recommended for use with conventional epifluorescent microscopes. It is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. Alexa Fluor® 647 conjugates are less expensive alternatives to allophycocyanin conjugates for flow cytometry.

Target: RatHost: GoatAntibody Format: Whole IgG Specificity: IgG, Fcγ fragment specificConjugate: Alexa Fluor® 647Product Category: Whole IgG Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2338395
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Jackson ImmunoResearch Laboratories,INC.专业致力于亲和纯化的二抗和免疫球蛋白的生产及偶联。产品主要销往世界各地的大学和科研院所。该公司位于宾夕法尼亚州费城以西约40英里,始建于1982年,经过40年左右的发展,Jackson的二抗以种类丰富(物种齐全,标记多样,应用广泛)、性价比高、供货迅捷而受到更多科研学者和工业客户的青睐。
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Jackson ImmunoResearch Laboratories,INC.专业致力于亲和纯化的二抗和免疫球蛋白的生产及偶联。产品主要销往世界各地的大学和科研院所。该公司位于宾夕法尼亚州费城以西约40英里,始建于1982年,经过40年左右的发展,Jackson的二抗以种类丰富(物种齐全,标记多样,应用广泛)、性价比高、供货迅捷而受到更多科研学者和工业客户的青睐。

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