WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculemouseIgG.Italsoreactswiththelightchainsofothermouseimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodyexhibitsinherentminimalcross-reactiontoratserumproteinsandhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithhuman,bovine,horse,rabbitandratserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState:Freeze-driedsolid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.


Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:100-1:800
FlowCytometry:-1:100-1:800

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

AlexaFluor®594

Amax:591Emax:614nm

AlexaFluor®594-conjugatedantibodiesabsorblightmaximallyaround591nmandfluorescewithapeakaround614nm.Theyarebrighter,morephotostable,andmorehydrophilicthanTexasRedconjugates.AlexaFluor®594conjugatesarebrighterthanred-fluorescingconjugates,andtheyprovidemorecolorseparationfromgreen-fluorescingdyesthanDyLight549,Cy3,andTRITCconjugates.Theyarethebestchoiceforimmunofluorescencedetectioninthedeep-redregionofthevisiblespectrum.