Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Storage: Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day.
Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative:None
Suggested Working Concentration or Dilution Range:
ELISA:- 10-20 µg / ml
Western Blot:- 10-20 µg / ml
Histo-/Cyto-Chemistry:- 10-20 µg / ml
Flow Cytometry:- 10-20 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.