Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Jackson冻干粉二抗如何配置
先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。