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主营:Jackson、二抗、血清、驴血清以及免疫球蛋白的生产和偶联
Jackson/AMCA AffiniPure Rabbit Anti-Rat IgG + IgM (H+L)/1.0 mg/312-155-048
产品编号:312-155-048
市  场 价:¥2820.00
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产品分类: 抗体类>二抗>抗大鼠>
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美  元  价:$141.00
品      牌: Jackson
公司分类:
Jackson/AMCA AffiniPure Rabbit Anti-Rat IgG + IgM (H+L)/1.0 mg/312-155-048
商品介绍

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with both rat IgG and IgM.It also reacts with the light chains of other rat immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

Coumarin AMCA

Amax: 350Emax: 450nm

Aminomethylcoumarin Acetate (AMCA) conjugates absorb light maximally around 350 nm and fluoresce maximally around 450 nm. For fluorescence microscopy, AMCA can be excited with a mercury lamp and observed using a UV filter set. Since blue fluorescence is not well detected by the human eye, AMCA-conjugated secondary antibodies should be used only with the most abundant antigens in multiple-labeling experiments. Ways of improving the visibility of AMCA include dark adapting the eyes, using fluorite instead of glass objectives, avoiding mounting media that absorb UV light (such as plastic-based media), and capturing photographic images with blue-sensitive film or CCD cameras. AMCA fades rapidly in conventional epifluorescence and confocal microscopy, and therefore it should be used with mounting media containing an anti-fading agent such as n-propyl gallate.

Target: RatHost: RabbitAntibody Format: Whole IgG Specificity: IgG + IgM (H+L)
Minimal Cross Reactivity: Human Serum Proteins
Conjugate: Coumarin AMCAProduct Category: Whole IgG Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_2339860
品牌介绍

Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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