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主营:Jackson、二抗、血清、驴血清以及免疫球蛋白的生产和偶联
Jackson/Rhodamine Red™-X (RRX) AffiniPure Fab Fragment Donkey Anti-Rat IgG (H+L)/1.0 mg/712-297-003
产品编号:712-297-003
市  场 价:¥2440.00
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产品分类: 抗体类>二抗>抗大鼠>
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美  元  价:$122.00
品      牌: Jackson
公司分类:
Jackson/Rhodamine Red™-X (RRX) AffiniPure Fab Fragment Donkey Anti-Rat IgG (H+L)/1.0 mg/712-297-003
商品介绍

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule rat IgG.It also reacts with the light chains of other rat immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Rhodamine Red™-X (RRX)

Amax: 570Emax: 590nm

RRX (Rhodamine Red-X) conjugates have a peak of excitation at 570 nm and a peak of emission at 590 nm. Although TRITC has been used traditionally with FITC for double labeling, better color separation is achieved by using RRX or Alexa Fluor® 594. Rhodamine Red-X is particularly useful for 3- and 4-color labeling with DyLight 405, Alexa Fluor® 488, and Alexa Fluor® 647 by using a confocal microscope equipped with a 405 nm laser and a krypton/argon laser. Fluorescence from RRX lies about midway between that of Alexa Fluor® 488 and Alexa Fluor® 647, and it shows little overlap with either dye. The krypton-argon laser emits lines at 488 nm, 568 nm, and 647 nm, which are optimal for exciting Alexa Fluor® 488, RRX, and Alexa Fluor® 647, respectively. By adding a 405 nm laser and a 420 nm emission filter, 4-color labeling is possible using DyLight 405-conjugated secondary antibodies from JIR (Figure 5). The separation between all four dyes is perfect for 4-color labeling, and all four dyes are very bright.

Target: RatHost: DonkeyAntibody Format: Fab Fragment Specificity: IgG (H+L)Conjugate: Rhodamine Red™-X (RRX)Product Category: Fab Fragment Affinity‑Purified AntibodiesClonality: Polyclonal RRID: AB_2340679
品牌介绍

Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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