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主营:Jackson、二抗、血清、驴血清以及免疫球蛋白的生产和偶联
℡ 4000-520-616
℡ 4000-520-616
Jackson/PerCP AffiniPure F(ab
产品编号:112-126-071
市  场 价:¥5360.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗大鼠>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$268.00
品      牌: Jackson
公司分类:
Jackson/PerCP AffiniPure F(ab
商品介绍
Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins.No antibody was detected against rat IgM or non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species.

F(ab")2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab")2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Store at 2-8°C – do not freeze. Prepare working dilution on day of use. Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.Fc fragments and whole IgG molecules have been removed. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:Flow Cytometry:- 1:25-1:100 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.Conjugate

PerCP

Amax: 488Emax: 675nm

PerCP is a fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. We offer the form found in Dinophyceae sp. with a molecular weight of about 35.5 kDa. It has a broad spectrum of excitation with a main peak at 482 nm, and a long Stokes shift to an emission peak at 677 nm.

PerCP, Alexa Fluor® 488 (or FITC), and R-PE are excited at 488 nm with an argon laser, and thus can be used for one-, two-, and three-color analyses with single-laser flow cytometers. APC and Alexa Fluor® 647 are excited at 633 nm to give a fourth color with dual-laser flow cytometers.

PerCP is also highly water soluble, has arelatively low isoelectric point, and lacks potentially sticky carbohydrates.

It should be noted that the relatively high molecular weight of PerCP may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

Images & References

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

品牌介绍

Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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