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Jackson/Cy™3 AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L)/1.0 mg/115-167-003
产品编号:115-167-003
市  场 价:¥3140.00
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产品分类: 抗体类>二抗>抗小鼠>
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美  元  价:$157.00
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Jackson/Cy™3 AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L)/1.0 mg/115-167-003
商品介绍

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Cyanine Cy™3

Amax: 550Emax: 570nm

Cy3 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. We recommend Cy3 as a brighter alternative to TRITC. Cy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy3 has been used with fluorescein for double labeling; however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize Cy3 fluorescence in the FITC filter set. Cy3 can also be paired with Alexa Fluor® 647 for multiple labeling when using a confocal microscope. However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor® 488) and a far-red-fluorescing dye like Alexa Fluor® 647.

Target: MouseHost: GoatAntibody Format: Fab Fragment Specificity: IgG (H+L)Conjugate: Cyanine Cy™3Product Category: Fab Fragment Affinity‑Purified AntibodiesClonality: Polyclonal RRID: AB_2338709
品牌介绍

Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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