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主营:Jackson、二抗、血清、驴血清以及免疫球蛋白的生产和偶联
℡ 4000-520-616
℡ 4000-520-616
Jackson/Cy™5 Streptavidin/1.0 mg/016-170-084
产品编号:016-170-084
市  场 价:¥3660.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$183.00
品      牌: Jackson
公司分类:
Jackson/Cy™5 Streptavidin/1.0 mg/016-170-084
商品介绍
Information Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:

Western Blot:- 0.5-2 µg / mlHisto-/Cyto-Chemistry:- 0.5-2 µg / mlDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Cyanine Cy™5

Amax: 650Emax: 670nm

Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy5 can be used with a variety of other fluorophores for multiple labeling due to a wide separation of its emission from that of shorter wavelength-emitting fluorophores. However, we recommend Alexa Fluor® 647 as the preferred far-red-fluorescing dye because it is brighter than Cy5 in aqueous mounting media. The reverse is true when mounting in non-polar, plastic mounting media, i.e. Cy5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor® 647 and DyLight 650.

Images & References
Above

Jejunum villi superficial epithelium labeled with Ulex-biotin Streptavidin-Cy5 (016-170-084 blue), gastric K cells labeled with goat anti-GIP + donkey anti-goat-Cy2 (705-225-147 green) and nerve fibers labeled with mouse anti-tubulin + donkey anti-mouse-Cy3 (715-165-151 red). Brian McAdams and William Kennedy, University of Minnesota.


Below

Vascular-associated astrocytes in the young adult rat retina

Tissue specimen of 3 month old (young adult) Wistar rat retina illustrates vascular-associated astrocytes in the mid-peripheral retina. Triple label immunohistochemistry was executed on retinal wholemount preparations. GFAP stained for astrocytes (red, Cy3-conjugated mouse monoclonal anti-GFAP), GS Isolectin B4 stained for blood vessels (blue, Cy5-conjugated streptavidin - Jackson ImmunoResearch 016-170-084), and S-100 stained for cell soma region of astrocytes (green, Alexa 488-conjugated anti-rabbit IgG).

Mansour, H., Chamberlain, C. G., Weible II, M. W., Hughes, S., Chu, Y. and Chan-Ling, T. (2008), Aging-related changes in astrocytes in the rat retina: imbalance between cell proliferation and cell death reduces astrocyte availability. Aging Cell, 7: 526–540. doi: 10.1111/j.1474-9726.2008.00402.x


This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Cy is a trademark of GE Healthcare Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by GE Healthcare Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.

品牌介绍

Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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