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主营:Jackson、二抗、血清、驴血清以及免疫球蛋白的生产和偶联
℡ 4000-520-616
℡ 4000-520-616
Jackson/Alkaline Phosphatase Streptavidin/1.0 mg/016-050-084
产品编号:016-050-084
市  场 价:¥3900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$195.00
品      牌: Jackson
公司分类:
Jackson/Alkaline Phosphatase Streptavidin/1.0 mg/016-050-084
商品介绍
Information Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.Usage Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended storage: Add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from the date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Buffer: 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:ELISA:- 1-2 µg / mlWestern Blot:- 0.1-1 µg / mlHisto-/Cyto-Chemistry:- 1-2 µg / mlDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.Conjugate

Alkaline Phosphatase

Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.

Images & References

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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Jackson冻干粉二抗如何配置

先加入蒸馏水稀释至Jackson二抗说明书所述浓度,再用二抗稀释液稀释至该二抗的建议稀释范围。


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