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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

The CellTiter 96 AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96 AQueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution.

Assays are performed by adding a small amount of the CellTiter 96 AQueous One Solution Reagent directly to culture wells, incubating for 1 4 hours and then recording absorbance at 490nm with a 96-well plate reader. The quantity of formazan product as measured by the amount of 490nm absorbance is directly proportional to the number of living cells in culture.

If you currently use a [3H]-thymidine incorporation assay, addition of the CellTiter 96 AQueous One Solution Reagent can be substituted for the pulse of [3H]-thymidine at the time point in the assay when the pulse of radioactive thymidine is usually added. Previous bioassay data comparing [3H]-thymidine incorporation to the MTS-based CellTiter 96 AQueous Assay and the original MTT-based CellTiter 96 Assay demonstrate that tetrazolium reagents can be substituted for [3H]-thymidine incorporation.

Features and Benefits

The simple \"add-incubate-measure\" format (single-step reagent addition) enables design of homogeneous high-throughput screening assays. Unlike MTT, the assay can be used as a single solution and is filter sterilized and ready to add to assay plates. Fewer steps are needed as the assay can be performed in 96-well plates with no washing or cell harvesting steps. Additionally, solubilization steps normally required for MTT assays are eliminated.

The assay provides flexibility as plates can be read and returned to an incubator for further color development, and with the elimination of volatile organic solvent to solubilize the formazan product and radioactive reagents commonly required of similar assays, the CellTiter 96 AQueous One Solution Cell Proliferation Assay is safer to use.

我们使用这些cookies以确保我们的网站安全及正常运转；它们是我们服务功能中所必需的，且不能在我们的系统中关闭。它们通常只在您有操作需求时才会响应，例如登录账号、使用购物车或填表时。关于这些cookies，您可以设置浏览器阻止或提醒，但如果没有它们的话我们将无法提供部分服务。与我们使用的其他cookies一样，绝对必需的cookies既可能是第一方cookies也可能是第三方cookies。

有关偏好的cookies

我们使用这些cookies来记住您的设置和偏好。譬如，我们可能会使用这些cookies来记住您的语言偏好。

有关性能和统计的cookies

我们使用这些cookies来收集您与我们的互动信息，以此作为评判和改进我们服务的依据。譬如，我们可能会使用这些cookies来判断您是否在某一特定页面有过互动。

有关营销的cookies

我们和我们的广告合作商使用这些cookies投放跟您关联且有意义的广告，并且可以及时追踪我们广告投放活动的效果，无论是在我们的网站还是在其他网站或社交媒体上。