WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswiththeFcportionofratIgGheavychainbutnotwiththeFabportionofratimmunoglobulins.NoantibodywasdetectedagainstratIgMoragainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState:Freeze-driedsolid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.


Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

AlexaFluor®647

Amax:651Emax:667nm

AlexaFluor®647-conjugatedantibodiesabsorblightmaximallyaround651nmandfluorescemaximallyaround667nm.TheyarebrighterthanCy5andDyLight650inaqueousmountingmedia.AlexaFluor®647-andAPC-conjugatedsecondaryantibodiesarethebestchoiceforflowcytometrywhensecondaryantibodiesfluorescingatthesewavelengthsaredesired.AlexaFluor®647conjugatesarethebestchoiceoffarred-emittingdyesformultiple-labelingdetectionwithaconfocalmicroscope.

AsignificantadvantageofusingAlexaFluor®647overlowerwavelength-emittingdyesisthelowautofluorescenceofBIOLOGicalspecimensinthisregionofthespectrum.However,becauseofitspeakemissionat667nm,AlexaFluor®647cannotbeseenwellbyeye,anditcannotbeexcitedoptimallywithamercurylamp.Therefore,AlexaFluor®647isnotrecommendedforusewithconventionalepifluorescentmicroscopes.Itismostcommonlyvisualizedwithaconfocalmicroscopeequippedwithanappropriatelaserforexcitationandafar-reddetector.AlexaFluor®647conjugatesarelessexpensivealternativestoallophycocyaninconjugatesforflowcytometry.